Enzymatic detection of Neisseria gonorrhoeae.

A study on the frequency of vaginal species of Mycoplasma genitalium, Gardnerella vaginalis and Neisseria gonorrhoeae among pregnant women by PCR technique

Bacterial vaginosis or non-specific vaginitis describes the disease caused by a change in the normal Flora of the vagina, which leads to the elimination of Lactobacilli, generating hydrogen peroxide and excess growth of bacteria, particularly anaerobic bacteria. This disease is the most prevalent infection of the female genital tract, and the rate of frequency of anaerobic bacteria, specificall...

شناسایی نایسریا گونورآ و کلامیدیا تراکوماتیس در بیماران مبتلا به اورتریت علامت دار با استفاده از روش های Multiplex PCR، رنگ آمیزی گرم و کشت ادرار در یاسوج

Background and purpose: Chlamydia trachomatis and Neisseria gonorrhoeae are the most common causes of sexually transmitted infections. The aim of this study was detection of N. gonorrhoeae and C. trachomatis in patients with symptomatic urethritis using Multiplex PCR, gram stain and urine culture. Materials and methods: This cross sectional study was conducted in 137 patients with symptomatic ...

RealLine Neisseria gonorrhoeae Fla-Format ASSAY KIT FOR THE QUALITATIVE DETECTION OF NEISSERIA GONORRHOEAE DNA BY REAL-TIME PCR METHOD

Enhanced gonococcal antimicrobial surveillance in the era of ceftriaxone resistance: a real-time PCR assay for direct detection of the Neisseria gonorrhoeae H041 strain.

OBJECTIVES Recent emergence of the extensively drug-resistant Neisseria gonorrhoeae H041 strain in Japan raises concerns that gonorrhoea may soon become untreatable and emphasizes the need for enhanced surveillance. In this study we developed a real-time PCR assay for direct detection of the H041 strain. METHODS Two real-time PCR assays for detection of the penA gene of the H041 strain, H041-...

One-step PCR for the identification of multiple antimicrobial resistance in Neisseria gonorrhoeae.

One-step multiplex PCR was developed for the identification of gonococci and antimicrobial-resistant profiles. From forty Neisseria gonorrhoeae isolates, the penicillinase-producing N. gonorrhoeae (PPNG), the high-level tetracycline-resistant N. gonorrhoeae (TRNG), and the ciprofloxacin-resistant N. gonorrhoeae (CRNG) were successfully classified. Our method provides expediency and benefit to e...